Journal: Journal of Neuroinflammation

Article Title: Neuroprotective epi-drugs quench the inflammatory response and microglial/macrophage activation in a mouse model of permanent brain ischemia

doi: 10.1186/s12974-020-02028-4

Figure Lengend Snippet: Inflammatory and microglia/macrophage mRNA expression profile in the ipsilateral hemisphere 1 day after pMCAO. ( a-l ) Quantification of Il6, Nos2, Il1b, Fcgr3, Fcgr2b, Mrc1, Arg1, Ym1, Tgfb1 and Trem2 by qRT-PCR in the ipsilateral hemisphere 1 day after pMCAO. Following pMCAO, the expression of ( a ) Il6 , ( b ) Nos2 , ( c ) Il1b , ( f ) Mrc1 and ( h ) Ym1 was higher, and ( g ) Arg1 showed an upward trend, if compared to the sham vehicle group. Treatment with MS-275 and resveratrol promoted a mild reduction of the expression of ( a ) Il6, ( f ) Mrc1 and ( g ) Arg1 , and prevented the increase of ( b ) Nos2 , ( c ) Il1b and ( h ) Ym1 transcript levels. One-way ANOVA followed by Holm-Šídák test, °trend, * p <0.05, ** p <0.01 vs. sham vehicle; # trend, §§ p <0.001 vs. MCAO vehicle. Data (means ± s.e.m., n = 6) are expressed as fold changes over values obtained in ipsilateral hemisphere from sham operated mice.

Article Snippet: The following day, slices were incubated with the secondary antibody for 1h at RT in PBS containing 1% BSA and 0.3% Triton X-100: for iNOS, biotinylated goat anti-mouse (1:600, Vector laboratories #BA-9200, Burlingame, CA, USA), or for CD68 and MRC1, biotinylated goat anti-rat (1:600, Vector laboratories #BA-9401, Burlingame, CA, USA) followed by streptavidin-Alexa Fluor 594 (1:1500, ThermoFisher Scientific #S11227, Waltham, MA, USA).

Techniques: Expressing, Quantitative RT-PCR

Journal: iScience

Article Title: InVision: An optimized tissue clearing approach for three-dimensional imaging and analysis of intact rodent eyes

doi: 10.1016/j.isci.2021.102905

Figure Lengend Snippet:

Article Snippet: Goat polyclonal anti-Mrc1 , R and D Systems , Cat#Af2535; RRID: AB_2063012.

Techniques: Software